Advances in fluorescent proteins, live-cell imaging, and superresolution instrumentation have ushered in a brand new period of investigations in mobile biology, medication, and body structure. From the id of the fairway fluorescent protein within the jellyfish Aequorea victoria to the engineering of novel fluorescent proteins, The Fluorescent Protein Revolution explores the historical past, homes, and functions of those very important probes.
The publication first strains the background of fluorescent proteins and the revolution they enabled in mobile imaging. It then discusses fluorescent proteins with novel photophysical homes. The booklet additionally covers a number of state-of-the-art imaging functions. those comprise superresolution microscopy of mobile superb constructions, agonize microscopy to imagine protein interactions and cell-signaling actions within dwelling cells, photobleaching and photoactivation innovations to imagine protein behaviors, suggestions that make the most plant and algal photoreceptors to permit light-regulated keep an eye on of enzymatic actions, and the noninvasive imaging of tumor–host interactions in residing animals.
In colour all through, this publication provides the elemental rules and most recent advances within the box, together with the linked improvement of imaging ideas that take advantage of fluorescent proteins. it truly is available to a vast viewers, from optical imaging specialists to beginners desiring an advent to the field.
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